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Several experimental studies report that estradiol modifies neutrophil metabolic pathways and can both augment NETosis and reduce or alter ROS-mediated cytotoxicity depending on the stimulus and receptor contexta duality that likely reflects receptor subtype expression, local estrogen concentration, and interacting co-signals in tissue microenvironments [40]

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For in vitro analysis, differentiated human SGBS adipocytes were subjected to GPR146 knockdown using siRNA (ThermoFisher) or overexpression via doxycycline-inducible lenti-viral vectors expressing either GPP (control) or GPR146