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In these patients, it had been described a reduction of overall bacterial richness and evenness [66], with a decrease in Bacteroidetes and Proteobacteria phyla and an increase of the phyla Firmicutes and Fusobacteria [66]
Safety and Side Effects: Most side effects are mild and temporary
2.2.6 Cortisol For cortisol analyses serum was gained by centrifugation of blood (15 min, 15C, 1950 g, Varifuge 3.0, Heraeus, Hanau, Germany) after incubation for 30 min at room temperature and 30 min at 30C and then stored at -80C until measurement
Potential Approaches: Extend assessment period (some effects develop gradually) Increase dose incrementally (add 50-100mcg weekly) Verify peptide quality and storage conditions Review administration technique Consider individual sensitivity factors Assess realistic expectations for research timeline Injection Site Issues Problem: Persistent redness, swelling, or discomfort at injection sites
Drugs and placebo were given for 6 months
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