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The eluted compounds were detected using LCMS-8030As LIQUID CHROMATOGRAPH MASS SPECTROMETER-Triple Quad MS (Waters Corporation, Milford, MA, USA) supplied with an electrospray ionization source (ESI) running in positive ionization mode to generate [M + H] + ion at m/z 162.23, 176.21 for nicotine and cotinine, respectively

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Plasma cotinine concentrations 30 nmol/l were found in 94% of self-reported daily smokers, 66% of occasional smokers, and 2% of nonsmokers

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