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Description

FAD is then fully reduced to FADH 2 upon nucleophilic attack by Cys379 on the disulfide linkage between Cys201 and the FAD adduct, thereby reforming the initial trisulfide bridge

Alternative medicine review : a journal of clinical therapeutic

B12, Biotin) 10 minutes per session

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To favor the recombination event and recovery of recombinants, the transformation mixtures were incubated overnight in liquid medium without antibiotic and in the presence of AdoCbl, since MetH requires B12 as cofactor