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Feeder cells were seeded with 1.25 10 4 cells/well in a 96-well plate and cultured for 1 day in the medium including bFGF and Y-27632, and iPS cells were additionally seeded with 5000 cells/well and cultured for 1 day

The food and drinking water were sterilized

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doi: 10.3389/fnagi.2025.1737001 Received 31 October 2025 Revised 13 December 2025 Accepted 17 December 2025 Published 10 February 2026 Volume 17 - 2025 Edited by Slawomir Jakiela, Warsaw University of Life Sciences, Poland Reviewed by Sajad Fakhri, Kermanshah University of Medical Sciences, Iran Emre Adigzel, Karamanolu Mehmetbey University, Trkiye Updates Copyright 2026 Guo and Chi