Quantitative in vitro to in vivo extrapolation (QIVIVE) of atrazine's effects on phosphodiesterase and aromatase mRNA expression in vitro to the rat in vivo based on pharmacokinetic
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In an ice water bath, 30L of blood sample was placed in a 96 deep-well plate, 100 L of ultrapure water was added, 500 L of precipitant (methanol) was added, the plate was sealed, vortexed and shaken for 10 min, and centrifuged (4000 rpm) for 15 min