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That is why the 2.0 mL reconstitution is recommended for 5mg vials

doi: 10.1159/000315162 248 ZhangWChenXYanZChenYCuiYChenBet al

doi: 10.1016/j.ejphar.2024.176629 123 NiuKBaiPYangBFengXQiuF

After removing the spent medium, cells were incubated in 500 l complete MEM containing antibiotics (10 g ml 1 Rif, 50 g ml 1 Mox or 20 g ml 1 Van) with or without 0100 M KL1 or its analogues (KL27), 20 M BHA (Sigma) or 010 M BIX-01294, in the presence of 50100 g ml 1 Gen at 37 C for 624 h