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The mixture was sonicated at 35C for 1 min followed by centrifugation at 8,000 rpm for 15 min at 40C

While human studies remain scarce, emerging in vitro models simulating human gut conditions reveal variable microbiota responses to MPs, ranging from dysbiosis and inflammatory metabolite production to probiotic enrichment and metabolic adaptation (Fournier et al., 2023

J Clin Invest 101(5):1012-9, 1998

Supernatants were collected and stored at 20C for insulin analysis

The use of more complex techniques as the one-path and the two-path impedance spectroscopy is able to measure the two components of the R epi , the R para and the R trans (R epi = R para + R trans ) on top of the R bas (28)