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5 M MOPS (pH 7
Transcription-based amplification methods (e
High efficiency cells result in high colony counts
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Cells were inoculated to an initial OD600 of ~0
OverExpress-C43(DE3) pLysS Chemically Competent Cells (SOLOs) BL21(DE3) 5 M MOPS (pH 7OverExpress C41(DE3) and C43(DE3) Competent Cells Express genes cloned into any T7 vector with these BL21(DE3) derivatives Effective in expressing toxic & membrane proteins Recommended for membrane proteins with T7 vector E. coli BL21(DE3) strains, like Lucigens E. cloni EXPRESS Competent Cells provide reliable expression of many genes cloned into T7 expression vectors (e. g., pET or Lucigens pSMART cDNA vectors). However, in some cases expression is