anti- actin mAb (MP Biomedicals, Irvine, CA., 110,000 dilution)
Monitoring during any protocol should include twice-weekly fasting glucose checks and serum IGF-1 every four weeks to confirm that levels remain within two standard deviations of the age-normalised range
Methylation reactions often occur with compounds containing hydroxyl (-OH), amine (-NH2), or thiol (-SH) functional groups
In brief, 3,000 cells were seeded in a 96-well Seahorse culture plate, with six wells included per biological replicate
Molecular mechanisms promoting cyclophosphamide-induced ovarian injury CP induces ovarian damage primarily through oxidative stress, inflammation, and apoptosis