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Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample

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Patients seeking B12 testing or treatment should consult their GP, who can arrange appropriate blood tests (serum B12, full blood count, folate, and if indicated, intrinsic factor and parietal cell antibodies) and prescribe treatment if deficiency is confirmed

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Some individuals experience mild gastrointestinal effects at higher doses