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In a randomized clinical study involving 75 women, sublingual Epitalon was administered at a dose of 0.5 mg/day for 20 days, with placebo and control cohorts for comparison

Global gene expression profiling of HLCs derived from differentiation Methods 1 and 2 was conducted using GeneChip PrimeView Human Gene Expression Arrays (Affymetrix

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Mitochondrial staining and reactive oxygen species (ROS) detection For mitochondrial staining, after washing once with PBS, 5 mM MitoTracker (Thermo Fisher Scientific) and 200 mM Hoechst (Thermo Fisher Scientific) were added to the cells