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When the cells reached suitable confluence, they were dissociated with trypsin (Gibco) and seeded into six-well plates

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Bullen said that while the proportion of participants who quit was highest in the e-cigarettes group - at 7.3 percent compared to 5.8 percent on nicotine patches and 4.1 percent on placebo - the differences were not statistically significant, so the results were that the two products were comparable