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The short halflife (~30 minutes) produces discrete GH pulses that mimic the bodys natural secretion pattern [1] [4]

Do not spray directly onto the lyophilised powder

The dissociation progress was checked under a phase contrast microscope at 4 magnification and the 37 C incubation was repeated in 5 min intervals, followed by trituration, until the organoids had dissociated into mostly single cells with a few remaining small clusters of cells (typically a total of 2530 min at 37 C)

Cell Death Differ (2020) 27(3):106785