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Details of the PCR amplification and primer have been previously described ( HLA antibody elution from kidney graft biopsies Remnant fragments of frozen kidney graft biopsies were thawed at room temperature and processed for antibody acid elution as previously described ( Statistical analysis Data were described as the mean and standard deviation (sd) or median and interquartile range (IQR) if continuous and as count and percent if categorical

Fer-1 (Merck Millipore, #341494) and DFO (Selleckchem, #S5742) served as protection controls

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