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Nicotine itself can be detected for a short period, but most tests look for its metabolite cotinine, which can remain in the body for several days to over a week depending on use

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Mol Biol Evol 36:632 Harris RB, Sackman A, Jensen JD (2018) On the unfounded enthusiasm for soft selective sweeps II: examining recent evidence from humans, flies, and viruses

The results showed that the vast majority of the fungi were unique to the healthy cigar tobacco leaves, with only Lasiodiplodia being exclusive to moldy leaves